The Malaria antigen test kit (Immunochromatography) is a rapid chromatographic immunoassay for the qualitative detection of circulating plasmodium falciparum (P.f.) and Pan-malaria antigens (P.f., P.v., P.o. and P.m.) in whole blood.

Principle

The Malaria P.f/Pan Antigen Rapid Test Device is a qualitative, membrane based immunoassay for the detection of P.f. and Pan antigens in whole blood. The membrane is pre-coated with anti- HRP-II antibodies and monoclonal antibody is pan to the lactate dehydrogenase of Plasmodium species. During testing the whole blood specimen reacts with the dye conjugate, which has been pre-coated on the test device. The mixture then migrates upward on the membrane by capillary action, reacts with anti-histidine-rich Protein I(HRP-II)antibodies on the membrane on P.f. test line region and with anti-pan-LDH antibodies on the membrane on Pan Line region. If the specimen contains HRP-II or plasmodium-specific Pan LDH or both, a colored line will appear in P.f line region or pan line region or two colored lines will appear in P.f. line region and Pan line region. The absence of the colored lines in P.f. line region or Pan line region indicates that the specimen does not contain HRP-II and/or plasmodium-specific Pan-LDH. To serve as a procedure control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

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