Patty Tavatanakit and Jake Knapp carded a final round of 65 to win the Grant Thornton Invitational in Naples, Florida.
The pair combined for an eagle and five birdies for a seven-under par round on Sunday as they finished on 27 under at the PGA & LPGA Tour mixed event.
The third round was played in a new format for team play. In the 'modified fourball', both players teed off and then switched balls for their second shots and played that ball until holed. The lower score counted as the team score.
"I putted well this week and I feel like everything has just been fun," said Thailand's Tavatanakit of her combination with US player Knapp.
"I feel like our chemistry was really good, we just talked about random things. It worked really well."
Thailand's Atthaya Thitikul and Korean Tom Kim posted a third round of 64, with two birdies on the last two holes giving them a second-place finish on 26 under.
US pair Jennifer Kupcho and Akshay Bhatia shot an eagle on the 17th to put themselves on 26 under, but they bogeyed the 18th to finish a shot further back.
New Zealand's Lydia Ko and Australia's Jason Day, who won the inaugural staging of this tournament in 2023, carded a third round 67 to end the tournament on 20 under.