Hibernian believe "VAR made a serious error" to not refer the dismissal of a penalty claim to an on-field review in their 2-2 draw at Aberdeen on Saturday.
In the first half at Pittodrie, Jordan Obita's cross appeared to hit the outstretched arm of Dons defender Nicky Devlin in the home box.
Hibs say referee David Munro was told by the VAR that the on-field decision to not give a spot kick "was correct".
But Munro should have been sent to the pitch-side monitor, Hibs add.
"The club held meetings with the Scottish FA at Hampden [on Monday] to challenge the decision," a club statement reads.
"The governing body outlined that the referee department agreed the incident should have been referred by VAR for on-field review, and therefore an error was made.
"This is not the first occasion where the club has received an apology for VAR errors, and the club will continue to work with the Scottish FA regarding officiating in the Scottish game."
BBC Scotland have approached the SFA for a response.
In the same match, Aberdeen manager Neil Warnock also felt his side should have been awarded a penalty when Hibs goalkeeper David Marshall collided with striker Bojan Miovski in the box.
"I thought that was manslaughter," Warnock said.
"Bojan heads the ball, David poleaxes him. Somebody that knows the game of football would have given a penalty there. If that's anywhere on the pitch, it's a booking. I'm bewildered at that."