Chelsea defender Tomas Kalas scored the only goal of the game in the first half.

Nigeria also lost 2-1 to England at Wembley last weekend, while the Czechs - who failed to qualify for the World Cup - lost 4-0 to Australia in their previous friendly outing.

Nigeria will face Argentina, Croatia and Iceland in Group D in Russia.

Their opening game is against Croatia in Kaliningrad on 16 June.

Despite the defeat, there was some encouraging news for Nigeria as Leicester midfielder Wilfred Ndidi - who had been out since April with a hamstring injury - started the game.