The security forces have fired tear gas to clear demonstrators in the Malian capital Bamako who are demanding the resignation of President Ibrahim Boubacar Keïta.
Hundreds of protesters spent the night on the streets after holding a march on Tuesday.
An alliance of opposition and religious leaders is spearheading the protests against economic decline, alleged government corruption and a long-running jihadist conflict.
Regional efforts to bring an end to the crisis have failed, with the opposition refusing to compromise on their demand that the president resigns.